Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats

Introduction: Strongyloides venezuelensis is a nematode whose natural host rats. It used as model for the investigation of human strongyloidiasis caused by S. stercoralis. The latter neglected tropical disease in Ecuador where there are no specific plans to mitigate this parasitic illness.Objective: To evaluate stages an experimental life cycle using Wistar rats.Materials and methods: Male rats were replicate biological describe its morphometric characteristics, well development. Furthermore, production eggs per gram feces was quantified two diagnostic techniques assessment parasite load: Kato-Katz qPCR.Results: Viable larval (L1, L2, L3) could be obtained up 96 hours through fecal culture. Parthenogenetic females established duodenum on fifth day postinfection. Fertile observed intestinal tissue fresh peak occurred 8th. post-infection. Unlike Kato-Katz, qPCR detected DNA days not typically reported.Conclusions: migration within murine environment equivalent that described cycle. quantitative technique showed quick low-cost, but had greater precision. This can tool study or other similar nematodiasis.